A simple, rapid, precise, accurate and reproducible high performance liquid chromatographic method was developed for estimation of loteprednol etabonate in eye drops. Chromatographic separation of the drug was achieved on a bondapak C-18 (300 × 3.9 mm, 5µ) column (waters) using a mobile phase, methanol and water in the ratio of 80:20% v/v, at a flow rate of 1 ml/min. The detection was carried out at 234 nm. The drug was eluted at the retention time of 5.3±0.1 min. The calibration curve was linear over the range of 5 µg – 600 μg/ml. The performance of the method was validated according to ICH guidelines. The method can be applied for determination of drug in its formulation with good accuracy and precision.
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